Difference between revisions of "Part:BBa J23094"
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this is a ser2 derived tRNA key with an addressive sequence that anneals 0 bases past the 5' most part of j23077 lock's rbs' while still completely covering the rbs'. the 3' most base on the lock's rbs' will be called base (0). so key(0)'s 3'-most binding occurs at base (0) on the lock. key(n)'s 3' most binding occurs at base n. rbs' is the complement of the RBS in the lock's hairpin. | this is a ser2 derived tRNA key with an addressive sequence that anneals 0 bases past the 5' most part of j23077 lock's rbs' while still completely covering the rbs'. the 3' most base on the lock's rbs' will be called base (0). so key(0)'s 3'-most binding occurs at base (0) on the lock. key(n)'s 3' most binding occurs at base n. rbs' is the complement of the RBS in the lock's hairpin. | ||
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{{Template:Berk2006iGEM-J23094-96Notes |.]] | {{Template:Berk2006iGEM-J23094-96Notes |.]] | ||
{{Berk2006iGEM-J23094-96Notes}} | {{Berk2006iGEM-J23094-96Notes}} | ||
Revision as of 05:46, 15 November 2006
Ser2 tRNA key(-33)
this is a ser2 derived tRNA key with an addressive sequence that anneals 0 bases past the 5' most part of j23077 lock's rbs' while still completely covering the rbs'. the 3' most base on the lock's rbs' will be called base (0). so key(0)'s 3'-most binding occurs at base (0) on the lock. key(n)'s 3' most binding occurs at base n. rbs' is the complement of the RBS in the lock's hairpin.
{{Template:Berk2006iGEM-J23094-96Notes |.]]
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 31
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]