Difference between revisions of "Part:BBa J119369:Experience"

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===Applications of BBa_J119369===
 
===Applications of BBa_J119369===
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Below are images and measurements of promoters strengths for four variants of the pClone -35 Consensus cloned into it. E. coli strain JM109 was transformed with constructs that included the Psimp1 promoter variants cloned into pClone Red (BBa_J119137).  The images were taken of the resulting colonies on a UV box. The numbers below each image are the relative strengths of the Psimp1 variants measured with RFP fluorometry.
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The plates shows six mutant promoters compared to Ptac. The table shows the ratios of RFP expression for the mutants promoters to that of Ptac as measured using ImageJ.  
 
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[[File:Fall_2014_Genetics_pClones.png |500px|]]
 
[[File:Fall_2014_Genetics_pClones.png |500px|]]

Latest revision as of 13:54, 4 April 2015


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Applications of BBa_J119369

The plates shows six mutant promoters compared to Ptac. The table shows the ratios of RFP expression for the mutants promoters to that of Ptac as measured using ImageJ.

Fall 2014 Genetics pClones.png

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