Difference between revisions of "Part:BBa J119377:Design"
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===Source=== | ===Source=== | ||
| − | Synthetic oligonucleotides cloned into pClone Red (BBa_J119137). | + | Synthetic oligonucleotides cloned into [https://parts.igem.org/Part:BBa_J119137 pClone Red ](BBa_J119137). |
===References=== | ===References=== | ||
Revision as of 13:32, 27 March 2015
Psimp2 Promoter with Four Consensus Variants
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The consensus sequence from De Mey et al. shown below was used to generate a promoter that has a simple sequence, by using W=T, N=C, R=A, and D=C, but avoiding runs of 4 or more Gs or Cs. The resulting promoter was named Psimp1.
Source
Synthetic oligonucleotides cloned into pClone Red (BBa_J119137).