Difference between revisions of "Part:BBa J119376:Design"
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| − | Synthetic oligonucleotides. | + | Synthetic oligonucleotides cloned with BsaI Golden Gate Assembly into [https://parts.igem.org/Part:BBa_J119137 pClone Red ](BBa_J119137). |
===References=== | ===References=== | ||
De Mey M, Maertens J, Lequeux GJ, Soetaiert WK, Vandamme EJ (2007) Construction and model-based analysis of a promoter library for E. coli: an indispensable tool for metabolic engineering. MC Biotechnol 7:34. | De Mey M, Maertens J, Lequeux GJ, Soetaiert WK, Vandamme EJ (2007) Construction and model-based analysis of a promoter library for E. coli: an indispensable tool for metabolic engineering. MC Biotechnol 7:34. | ||
Latest revision as of 13:33, 27 March 2015
Psimp1 Promoter with Four Consensus Variants
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The consensus sequence from De Mey et al. shown below was used to generate a promoter that has a simple sequence by using W=A, N=G, R=G, and D=G, but avoiding runs of 4 or more Gs or Cs. The resulting promoter was named Psimp1.
Source
Synthetic oligonucleotides cloned with BsaI Golden Gate Assembly into pClone Red (BBa_J119137).
References
De Mey M, Maertens J, Lequeux GJ, Soetaiert WK, Vandamme EJ (2007) Construction and model-based analysis of a promoter library for E. coli: an indispensable tool for metabolic engineering. MC Biotechnol 7:34.