Difference between revisions of "Part:BBa J100198:Experience"
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This part works as designed. thyA- cells that contain J100198 do not grow unless theophylline is added to the media. Growth rates are comparable with 0.3, 0.6 and 1.25 mM theophylline added to LB broth + 50 µg/mL ampicillin (to select for pSB1A8 plasmid) + 50 µg/mL kanamycin (to select for thyA- mutation). | This part works as designed. thyA- cells that contain J100198 do not grow unless theophylline is added to the media. Growth rates are comparable with 0.3, 0.6 and 1.25 mM theophylline added to LB broth + 50 µg/mL ampicillin (to select for pSB1A8 plasmid) + 50 µg/mL kanamycin (to select for thyA- mutation). | ||
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[[File:cellsNtubes.png]]<br> | [[File:cellsNtubes.png]]<br> | ||
Photograph of ''thyA-'' cells that contain J100198 in pSB1A8 grown in LB broth + 50 µg/mL ampicillin (to select for pSB1A8 plasmid) + 50 µg/mL kanamycin (to select for thyA- mutation). From left to right: 0, 0.3, 0.6 and 1.25 mM theophylline. <br> | Photograph of ''thyA-'' cells that contain J100198 in pSB1A8 grown in LB broth + 50 µg/mL ampicillin (to select for pSB1A8 plasmid) + 50 µg/mL kanamycin (to select for thyA- mutation). From left to right: 0, 0.3, 0.6 and 1.25 mM theophylline. <br> | ||
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[[File:Cells_graphed..png]]<br> | [[File:Cells_graphed..png]]<br> | ||
Revision as of 13:39, 20 December 2014
This part works as designed. thyA- cells that contain J100198 do not grow unless theophylline is added to the media. Growth rates are comparable with 0.3, 0.6 and 1.25 mM theophylline added to LB broth + 50 µg/mL ampicillin (to select for pSB1A8 plasmid) + 50 µg/mL kanamycin (to select for thyA- mutation).
Photograph of thyA- cells that contain J100198 in pSB1A8 grown in LB broth + 50 µg/mL ampicillin (to select for pSB1A8 plasmid) + 50 µg/mL kanamycin (to select for thyA- mutation). From left to right: 0, 0.3, 0.6 and 1.25 mM theophylline.
OD590 of thyA- cells that contain J100198 in pSB1A8 grown in LB broth + 50 µg/mL ampicillin (to select for pSB1A8 plasmid) + 50 µg/mL kanamycin (to select for thyA- mutation) with added theophylline as indicated. The cells were grown overnight and measured then ext morning.
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Applications of BBa_J100198
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