Difference between revisions of "Part:BBa J100205"

Revision as of 17:52, 7 December 2014

repClone Red

We designed repClone Red to test the function of repressors. Internal BsaI sites allow the insertion of the pTet promoter to activate TetR. Variations on this promoter or introduction of tetracycline will affect the activity of the repressor, as indicated by the fluorescence of colonies containing the plasmid. This can be used in synthetic biology laboratories or for undergraduate introductory biology lab modules.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal SpeI site found at 9
12
INCOMPATIBLE WITH RFC[12]
Illegal SpeI site found at 9
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal SpeI site found at 9
25
INCOMPATIBLE WITH RFC[25]
Illegal SpeI site found at 9
Illegal AgeI site found at 2288
Illegal AgeI site found at 2400
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 1638
Illegal BsaI.rc site found at 1517

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 __NOTOC__
 <partinfo>BBa_J100205 short</partinfo>
-We designed repClone Red to test the function of repressors. The tetR repressor is present but inactive because pTet is not included. Addition of pTet will prevent translation of GFP. Addition of tetracycline to the environment (or anhydrotetracycline) will prevent inhibition by tetR.  This can be used in synthetic biology laboratories or for undergraduate introductory biology lab modules.
+We designed repClone Red to test the function of repressors. Internal BsaI sites allow the insertion of the pTet promoter to activate TetR. Variations on this promoter or introduction of tetracycline will affect the activity of the repressor, as indicated by the fluorescence of colonies containing the plasmid. This can be used in synthetic biology laboratories or for undergraduate introductory biology lab modules.
 <!-- Add more about the biology of this part here