Difference between revisions of "Part:BBa M36921"
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===Functional Parameters===
 
===Functional Parameters===
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This system will be placed into a Comet plasmid with a high-copy origin of replication and Kanamycin resistance as its selection marker. We observed that an increasing concentration of Zn2+ did correlate with an increasing amount of Comet fluorescence. However, due to a comparison with controls and further statistical analysis, the composite Zn sensor produced unspecific effects in relation to Zn2+ concentrations.
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<partinfo>BBa_M36921 parameters</partinfo>
 
<partinfo>BBa_M36921 parameters</partinfo>
 
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Revision as of 06:42, 5 December 2014

Zinc Sensor

This composite part contains a constitutive promoter, ribosome-binding-site, a gene for ZntR, an E. Coli terminator, and a promoter that is ZntR regulated. In the absence of Zinc ions, the ZntR protein binds to the ZntR promoter binding site. In the presence of zinc ions, ZntR undergoes a conformation, does not attach to the binding site, and allows for transcription of our actuator.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 194
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal PstI site found at 194
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 190
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 194
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 194
  • 1000
    COMPATIBLE WITH RFC[1000]