Difference between revisions of "Part:BBa M36938:Experience"
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'''Performance Data:'''
 
'''Performance Data:'''
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'''Protein Presence Test: GFP Fluorescence Reading'''
 
  
 
Note: The entries in the same numerical position in each list are data for one sample.
 
Note: The entries in the same numerical position in each list are data for one sample.
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'''Protein Presence Test: GFP Fluorescence Reading'''
  
 
''Condition'' GFP GFP GFP GFP Control Control Control Control Blank
 
''Condition'' GFP GFP GFP GFP Control Control Control Control Blank
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p=0.011066469 ---> p < 0.05
 
p=0.011066469 ---> p < 0.05
 
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Conclusion: The target protein (enoyl-[acyl-carrier-protein] reductase)causes an increase in pH and therefore is functional. This is statistically significant.
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Conclusion: Production of the target protein (enoyl-[acyl-carrier-protein] reductase) causes an increase in pH and therefore is functional. This is statistically significant.
  
 
===User Reviews===
 
===User Reviews===

Revision as of 06:04, 3 December 2014

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_M36938

Stanford Location:

Box Name: BIOE44 S14

Glycerol Freezer Stocks:

Barcode# :133011796, Plasmid Name: CME LCPUFA_Dasher, Resistance Type: amp, DNA 2.0 Gene #:193425, Organism: E. coli, Device Type: LCPUFA Actuator.

Barcode# : 133024687, Plasmid Name: CME LCPUFA_Dasher, Resistance Type: amp, DNA 2.0 Gene #:193425, Organism: E. coli, Device Type: LCPUFA Actuator.

Barcode# : 133010087, Plasmid Name: CME LCPUFA_Dasher, Resistance Type: amp, DNA 2.0 Gene #:193425, Organism: E. coli, Device Type: LCPUFA Actuator.

Barcode# : 133011361, Plasmid Name: CME LCPUFA_Dasher, Resistance Type: amp, DNA 2.0 Gene #:193425, Organism: E. coli, Device Type: LCPUFA Actuator.

Performance Data:

Note: The entries in the same numerical position in each list are data for one sample.

Protein Presence Test: GFP Fluorescence Reading

Condition GFP GFP GFP GFP Control Control Control Control Blank

OD600 1.44 1.377 1.422 1.377 1.432 1.448 1.45 1.451 0.037

GFP AFU 3537 4250 3725 3604 829 966 918 853 658

Standardized OD600 1.403 1.34 1.385 1.34 1.395 1.411 1.413 1.414 0

Standardized GFP AFU 2852 3565 3040 2919 144 281 233 168 0

AFU/OD600 2032.78689 2660.44776 2194.94585 2178.35821 103.225806 199.149539 164.897381 118.811881

GFP Average: 2266.63468 Control Average: 146.521152

p = 0.00036738 ---> p < 0.001

Conclusion: The target protein (enoyl-[acyl-carrier-protein] reductase) is synthesized within the E. coli cells. This result is statistically significant.


Protein Functionality Test: pH Reading

Standard(no GFP) pH Readings: 6.91 6.64 6.49 6.42 6.64 6.45 6.34 6.4

Control pH Readings:

6.56 6.49 6.42 6.36 6.25 6.26 6.29 6.34

Standard Average pH: 6.53625

Control Average pH: 6.37125

p=0.011066469 ---> p < 0.05

Conclusion: Production of the target protein (enoyl-[acyl-carrier-protein] reductase) causes an increase in pH and therefore is functional. This is statistically significant.

User Reviews

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