Difference between revisions of "Part:BBa J100202"
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− | We are building | + | We are building actClone Red to test inducers. This intermediate is a pSB1A8 plasmid containing RFP, RBS, and forward promoter. It will be an indicator of successful ligation of an inducer to the final plasmid. |
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Latest revision as of 18:18, 26 September 2016
Intermediate for IndClone Red
We are building actClone Red to test inducers. This intermediate is a pSB1A8 plasmid containing RFP, RBS, and forward promoter. It will be an indicator of successful ligation of an inducer to the final plasmid.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 805
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 805
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 805
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 805
Illegal AgeI site found at 678
Illegal AgeI site found at 790 - 1000COMPATIBLE WITH RFC[1000]