Difference between revisions of "Part:BBa K1499250"
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<!--This part is GFP with two inserted TAG stop codons in place of leucine residues. This part transformed into Amberless cells along with supP tRNA  will express GFP. In wild type cells, no GFP will be expressed as the TAG will be recognized as stop codons rather than leucine. This part is used as a proof of concept for Codon Security which mitigates the effects of horizontal gene transfer by making these genes unreadable by natural organisms.
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<div class="small-7 small-centered columns"><br><center><img src="https://static.igem.org/mediawiki/2014/a/a4/SBSiGEM_HellCell1.png"><br>
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<h6><b>Figure 1.</b> Our approach to the Amberless Hell Cell. By recoding the UAG stop codon to translate into an amino acid, only cells that have a tRNA with the anticodon AUC will produce the complete protein. In our experiment, we used a tRNA that charges with leucine to translate the UAG codon.</h6>
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===Usage and Biology===
 
===Usage and Biology===
  
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This part is GFP with two inserted TAG stop codons in place of leucine residues. This part transformed into Amberless cells along with supP tRNA  will express GFP. In wild type cells, no GFP will be expressed as the TAG will be recognized as stop codons rather than leucine. This part is used as a proof of concept for Codon Security as part of the Amberless Hell Cell project which mitigates the effects of horizontal gene transfer.
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[[File:SBSiGEM_HellCell1.png|700px|thumb|center|<b>Figure 2.</b> Our approach to the Amberless Hell Cell. By recoding the UAG stop codon to translate into an amino acid, only cells that have a tRNA with the anticodon AUC will produce the complete protein. In our experiment, we used a tRNA that charges with leucine to translate the UAG codon.]]
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K1499250 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1499250 SequenceAndFeatures</partinfo>

Revision as of 20:11, 2 November 2014

GFP (E0040) with two amber stop codons



Usage and Biology

This part is GFP with two inserted TAG stop codons in place of leucine residues. This part transformed into Amberless cells along with supP tRNA will express GFP. In wild type cells, no GFP will be expressed as the TAG will be recognized as stop codons rather than leucine. This part is used as a proof of concept for Codon Security as part of the Amberless Hell Cell project which mitigates the effects of horizontal gene transfer.

Figure 2. Our approach to the Amberless Hell Cell. By recoding the UAG stop codon to translate into an amino acid, only cells that have a tRNA with the anticodon AUC will produce the complete protein. In our experiment, we used a tRNA that charges with leucine to translate the UAG codon.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 705