Difference between revisions of "Part:BBa K1398004"

Revision as of 19:52, 2 November 2014

NemR Intergenic Reporter

A construct created to test the effectiveness of the NemR upstream intergenic region (BBa_1398005). The construct contains the entire NemR upstream region (BBa_1398005), which contains a promoter and RBS. It is followed by the fluorescent reporter iLOV (listed in the repository as BBa_K660004), a double STOP codon and a double terminator (B0015).

No fluorescence response was observed when TNT was introduced in solution with the plasmid carrying bacteria (004).

Flu-OF_compared_to_0ul_TNT_as_the_level_of_TNT_in_a_culture_changes..png

Further information of part 007 can be found here:

https://parts.igem.org/Part:BBa_K1398007

The full article detailing the characterisation of this part can be found here:

http://2014.igem.org/Team:Exeter/Detection

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 5: / Line 5: @@
 The construct contains the entire NemR upstream region (BBa_1398005), which contains a promoter and RBS. It is followed by the fluorescent reporter iLOV (listed in the repository as BBa_K660004), a double STOP codon and a double terminator (B0015).
-No fluorescence response was observed when TNT was introduced in solution with the plasmid carrying bacteria.
+No fluorescence response was observed when TNT was introduced in solution with the plasmid carrying bacteria (004).
 https://static.igem.org/mediawiki/parts/1/11/Flu-OF_compared_to_0ul_TNT_as_the_level_of_TNT_in_a_culture_changes..png
+Further information of part 007 can be found here:
+https://parts.igem.org/Part:BBa_K1398007
 The full article detailing the characterisation of this part can be found here: