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| | ===Applications of BBa_K1321343=== | | ===Applications of BBa_K1321343=== |
| − | As part of our project we carried out an assay to determine the relative binding ability of CBD-sfGFP and sfGFP fusions to bacterial cellulose.
| + | For reference, the cellulose binding domain binding capability of CBDclos (C-terminally fused) to bacterial cellulose was measured relative to other cellulose binding domains when fused to sfGFP, the data for which can be seen here (here) - K1321346. |
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| − | Our first assay was performed to determine the relative strengths of various CBDs’ binding to bacterial cellulose. These were represented by the percentage fluorescence left from CBDs fused to ([https://parts.igem.org/Part:BBa_K1321337 sfGFP (RFC25)]) bound to bacterial cellulose discs, when subjected to various washes. Full protocol can be found ([http://2014.igem.org/Team:Imperial/Protocols here])
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| − | In the same assay, results suggested that dCBD, CBDcex, CBDcipA, and C-terminal CBDclos (all fused to sfGFP) had greater ability to bind bacterial cellulose than N-terminal CBDclos. Three washes with dH2O, PBS and 5% BSA were carried out to monitor binding strength. It was determined that the fusion with N-terminal CBDclos was, on average, our fourth strongest CBD, in comparison other CBDs tested.
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| − | [[File:IC14_-_dH2Obplot1.png|600px|thumb|left|Figure 1 - CBD binding strength after three dH2O washes. ]]
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| − | [[File:IC14-EtOHbplot1.png|600px|thumb|left|Figure 2 - CBD binding strength after three 70%Ethanol washes]]
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| − | [[File:IC14-PBSbplot1.png|600px|thumb|left|Figure 3 - CBD binding strength after three PBS washes]]
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| − | [[File:IC14-BSAbplot1.png|600px|thumb|left|Figure 4 - CBD binding strength after three BSA washes]]
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| | ===User Reviews=== | | ===User Reviews=== |
Revision as of 05:48, 2 November 2014
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Applications of BBa_K1321343
For reference, the cellulose binding domain binding capability of CBDclos (C-terminally fused) to bacterial cellulose was measured relative to other cellulose binding domains when fused to sfGFP, the data for which can be seen here (here) - K1321346.
User Reviews
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