Difference between revisions of "Part:BBa K1332010"

Revision as of 04:49, 2 November 2014

mRNA circularization device (3� side)

This part consists of the 5' side of the intron(+exon fragment) from td gene of T4 phage(BBa_K1332004) and a double terminator(BBa_B0015). The protein coding sequence that is inserted between this device and mRNA circularization device (5’ side)(BBa_K1332008) can be circularized. The 5' side of the intron(+exon fragment) from td gene of T4 phage contains a stop codon, so a circular mRNA made by this part doesn’t cause continuous translation.

explanation of this part

Figure 1. mRNA circularization device (3'side)(not endless translation)

Part which surrounded by red closing line is mRNA circularization device (3’side)(not endless translation).(figure 1)

How to use

Insert the protein coding sequence between this device and mRNA circularization device (5’ side). How to use this part is same as how to use 5' side device(BBa_K1332008) or 3' side device(endless translation)(BBa_K1332009) . Thus,read that.

(BBa_K1332008)

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 11: / Line 11: @@
 <h3>How to use</h3>
-Insert the protein coding sequence between this device and mRNA circularization device (5’ side)([https://parts.igem.org/Part:BBa_K1332008 BBa_K1332008]).
+Insert the protein coding sequence between this device and mRNA circularization device (5’ side).
+How to use this part is same as how to use 5' side device([https://parts.igem.org/Part:BBa_K1332008 BBa_K1332008]) or 3' side device(endless translation)([https://parts.igem.org/Part:BBa_K1332009 BBa_K1332009]) .
+Thus,read that.
+([https://parts.igem.org/Part:BBa_K1332008 BBa_K1332008])
 <!-- Add more about the biology of this part here