Difference between revisions of "Part:BBa K1555000"
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Revision as of 00:14, 2 November 2014
File:Https://static.igem.org/mediawiki/parts/1/19/2h 0mg.L copper induce .tif The copa promoter wth a gfp reporter
This part is our testing part.The copa is one of the candidates of our copper sensor.For comparing with other two sequences,marO(BBa_K1555001) and cueo(BBa_K1555002),we linked it with a reporter(BBa_E0840).With the concentration of copper rises, the expression of downstream gfp coding gene increases.We designed two experiments to test those candidates' sensitivity and specificity individually by measuring fluorescence intensity. File:2h 0mg.Lcopper induce.tifFile:Part:BBa K1555000 Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 914