Difference between revisions of "Part:BBa K1391115:Experience"
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===Applications of BBa_K1391115===
 
===Applications of BBa_K1391115===
  
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In the brain of a patient with Alzheimer's disease, beta-amyloid protein oligomers accumulate into plaques, which are responsible for the degenerative symptoms of the disease. In order to diagnose Alzheimer's disease, this system uses beta-amyloid oligomer-specific, transmembrane receptors to detect the presence of beta-amyloid oligomers.
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Cofilin is an actin binding protein that has been shown to also act as a cofactor when interacting with the receptors LilrB2 and PirB. When these receptors are activated, they recruit cofilin, which then gets activated itself. Inactive cofilin is phosphorylated at the third amino acid and it gets activated by de-phosphorylation by the receptors. The sequence for this part encodes an amino acid at the third position that is different from naturally occurring cofilin. This provides a "pseudophosphrylated" effect that we hope to limit cross reactions between other components of the cell, increasing the orthogonality of our system.
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Human Leukocyte immunoglobulin-like receptor subfamily B member 2 (LilrB2) is a naturally occurring, transmembrane protein receptor that selectively binds beta-amyloid oligomers. LilrB2 belongs to a family of proteins that bind to MHC1 molecules on antigen presenting cells, and is only expressed in monocytes and B-cells (and at lower levels in dendritic cells and natural killer cells) in humans. When beta-amyloid oligomers bind to the extracellular domain of LilrB2, it becomes activated and recruits a protein called cofilin (found inside the cell) to its intracellular domain.  
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In this detection system, LilrB2 was fused to a linker, a TEV protease (TEVp) cleavage site and a transcription factor (in that order) at its intracellular domain. Cofilin was fused to TEV protease. These modifications allowed the manipulation of the natural operational system of LilrB2 such that when beta-amyloid oligomers bind to the receptor (and activate it) the TEV protease on the recruited cofilin cleaves at the TEVp cleavage site. This releases the transcription factor in to the cytosol, where it is guided to the nucleus of the cell and activates some subsequent (reporter or treatment) module.
 
In this detection system, LilrB2 was fused to a linker, a TEV protease (TEVp) cleavage site and a transcription factor (in that order) at its intracellular domain. Cofilin was fused to TEV protease. These modifications allowed the manipulation of the natural operational system of LilrB2 such that when beta-amyloid oligomers bind to the receptor (and activate it) the TEV protease on the recruited cofilin cleaves at the TEVp cleavage site. This releases the transcription factor in to the cytosol, where it is guided to the nucleus of the cell and activates some subsequent (reporter or treatment) module.
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The expression of this fusion protein can be induced using doxycycline via the inducible tetracycline responsive promoter.
  
  

Revision as of 21:24, 1 November 2014

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Applications of BBa_K1391115

Cofilin is an actin binding protein that has been shown to also act as a cofactor when interacting with the receptors LilrB2 and PirB. When these receptors are activated, they recruit cofilin, which then gets activated itself. Inactive cofilin is phosphorylated at the third amino acid and it gets activated by de-phosphorylation by the receptors. The sequence for this part encodes an amino acid at the third position that is different from naturally occurring cofilin. This provides a "pseudophosphrylated" effect that we hope to limit cross reactions between other components of the cell, increasing the orthogonality of our system.

In this detection system, LilrB2 was fused to a linker, a TEV protease (TEVp) cleavage site and a transcription factor (in that order) at its intracellular domain. Cofilin was fused to TEV protease. These modifications allowed the manipulation of the natural operational system of LilrB2 such that when beta-amyloid oligomers bind to the receptor (and activate it) the TEV protease on the recruited cofilin cleaves at the TEVp cleavage site. This releases the transcription factor in to the cytosol, where it is guided to the nucleus of the cell and activates some subsequent (reporter or treatment) module.

The expression of this fusion protein can be induced using doxycycline via the inducible tetracycline responsive promoter.


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