Difference between revisions of "Part:BBa K1539001:Experience"
Stassoulas3 (Talk | contribs) (→Applications of BBa_K1539001) |
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===Applications of BBa_K1539001=== | ===Applications of BBa_K1539001=== | ||
| − | BBa_K1539001 was created using devices XXX and YYY to insert relatively high expressing promoters and high efficiency RBS sites in front of mCherry. Expression of mCherry was quantified by flow cytometry using a FACS AriaIII. Relative to cells transfected with an mCherry plasmid without a promoter or RBS site, | + | BBa_K1539001 was created using devices XXX and YYY to insert relatively high expressing promoters and high efficiency RBS sites in front of mCherry from BBa_J0650. Expression of mCherry was quantified by flow cytometry using a FACS AriaIII. Relative to cells transfected with an mCherry plasmid without a promoter or RBS site, 91.6% of our cells expressed mCherry and the median intensity was 4X brighter than a low promoter/weak RBS combination (see part [https://parts.igem.org/wiki/index.php?title=Part:BBa_K1539002]). Our raw data is summarized below: |
| + | "High Expression Combination" - Primers PH1/RH1: | ||
[[File:file:///C:/Users/yannis/Downloads/140821_DMC_mCherry_PH1-RH1.pdf]] | [[File:file:///C:/Users/yannis/Downloads/140821_DMC_mCherry_PH1-RH1.pdf]] | ||
| + | Negative Control: | ||
===User Reviews=== | ===User Reviews=== | ||
Revision as of 20:30, 1 November 2014
This experience page is provided so that any user may enter their experience using this part.
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how you used this part and how it worked out.
Applications of BBa_K1539001
BBa_K1539001 was created using devices XXX and YYY to insert relatively high expressing promoters and high efficiency RBS sites in front of mCherry from BBa_J0650. Expression of mCherry was quantified by flow cytometry using a FACS AriaIII. Relative to cells transfected with an mCherry plasmid without a promoter or RBS site, 91.6% of our cells expressed mCherry and the median intensity was 4X brighter than a low promoter/weak RBS combination (see part [1]). Our raw data is summarized below:
"High Expression Combination" - Primers PH1/RH1: File:File:///C:/Users/yannis/Downloads/140821 DMC mCherry PH1-RH1.pdf Negative Control:
User Reviews
UNIQce64890b9863ed5c-partinfo-00000000-QINU UNIQce64890b9863ed5c-partinfo-00000001-QINU