Difference between revisions of "Part:BBa K1360002"
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This part is the coding sequence for a thermostable xylanase, which specifically degrades xylan. | This part is the coding sequence for a thermostable xylanase, which specifically degrades xylan. | ||
− | + | [[File:XynB_decolorfour.png|thumb|]] | |
===Usage and Biology=== | ===Usage and Biology=== | ||
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[[File:DNS_Synergy5.png|center|frame||As you can see, the real DNS color is darker, so we think they can work in synergy.]] | [[File:DNS_Synergy5.png|center|frame||As you can see, the real DNS color is darker, so we think they can work in synergy.]] | ||
+ | [[File:DNS_Absorbance.png|center|frame||We also collect the absorbance data of the DNS. In this DNS method, OD540 corresponds to reductive saccharide concentration, but due to equipment limitation, we used OD570 for plate reader data.]] | ||
+ | ===Extra=== | ||
+ | [[File:Decolorfour.png|center|frame|In 2014, team Tongji's project Decolor Four.]] | ||
+ | [[File:Principle_Bleaching.gif|center|frame||The reason why these three hemicellulase can reduce chlorine consumption is because they break down hemicellulose which connects lignin and cellulose. In this way, bleaching detergent can "get into" the pulp easier, and less chlorine can make the same effect. This benefits environmental protection.]] | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> |
Latest revision as of 04:37, 2 November 2014
endo-1,4-beta-xylanase XynB
This part is the coding sequence for a thermostable xylanase, which specifically degrades xylan.
Usage and Biology
The XynB enzyme produced by this part can be used in pulp bleaching. It presents high activity under a wide range of temperature (55℃-99℃) and pH conditions (3-10.5). The optimum working condition is pH = 6.0, temperature = 65.9℃. This enzyme is expressed at high level in E.coli BL21(DE3) strain after T7 promoter.
Design considerations
This part is only a coding sequence without expression regulators, and is compatible with RFC10 standards, and the codon is optimized for E.coli expression.
Lab Archives
Extra
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 847
Illegal AgeI site found at 610 - 1000COMPATIBLE WITH RFC[1000]