Difference between revisions of "Part:BBa K1419003"
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Universal RNA-IN RBS part | Universal RNA-IN RBS part | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
Allows for the riboregulation of translation of proteins expresses using this RBS. Based off of the Elowitz RBS (B0034) | Allows for the riboregulation of translation of proteins expresses using this RBS. Based off of the Elowitz RBS (B0034) | ||
Latest revision as of 18:45, 1 November 2014
Universal riboregulatable RBS
Universal RNA-IN RBS part
Usage and Biology
Allows for the riboregulation of translation of proteins expresses using this RBS. Based off of the Elowitz RBS (B0034)
Contains the S32 RNA-IN sequence from Mutalik et. al. (2012) spaced appropriately upstream from the RBS. Downstream of the RBS is a start codon and the RFC 25 suffix. When assembled with a protein coding part, there will be an addition of 6 amino acids to the N-terminus. This is necessary to allow for a single RNA-OUT molecule to regulate any protein expressed using this RBS. Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 32
- 1000COMPATIBLE WITH RFC[1000]