Difference between revisions of "Part:BBa K1360001"
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<partinfo>BBa_K1360001 short</partinfo>
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a gene encoding thermostable alpha-L-arabinofuranosidase from ArfB of Clostridium stercorarium DSM8532, codon optimized for E.coli
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Xylanases are enzymes that degrad Xylan, a major component of wood. This is the circular counterpart of <partinfo>BBa_K1362020</partinfo>. A circular Xylanase should be resistent to exo-peptidases and could be more heat stable because of its reduced conformational space. This could be interesting for industrial applications, as they are often performed at higher temperatures and harsher environments.
  
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<!-- Add more about the biology of this part here
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[[File:DNS_Abstract.png|thumb|caption]]
 
===Usage and Biology===
 
===Usage and Biology===
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To characterize our part in respect to its thermal stability we conducted a Heatshock Assay. Firstly the Xylanases were incubated at 37 °C, 50 °C, 56 °C, 60 °C and 66 °C. After 30 minutes substrate (EnzChek® Ultra Xylanase Assay Kit) was added and the fluorescence was measured for 150 minutes in a platereader at 37 °C. The results indicate a reduction of activity at temperatures higher than 50 °C and complete loss of function for temperatures higher than 60 °C.
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K1360001 SequenceAndFeatures
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<partinfo>BBa_K1362022 SequenceAndFeatures</partinfo>
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<img src=https://static.igem.org/mediawiki/2014/f/f6/Paperpulp3.png>
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</partinfo>
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===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_K1360001 parameters</partinfo>
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<partinfo>BBa_K1362022 parameters</partinfo>
 
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Revision as of 13:11, 1 November 2014

No argument specified with partinfo tag.

Xylanases are enzymes that degrad Xylan, a major component of wood. This is the circular counterpart of BBa_K1362020. A circular Xylanase should be resistent to exo-peptidases and could be more heat stable because of its reduced conformational space. This could be interesting for industrial applications, as they are often performed at higher temperatures and harsher environments.

caption

Usage and Biology

To characterize our part in respect to its thermal stability we conducted a Heatshock Assay. Firstly the Xylanases were incubated at 37 °C, 50 °C, 56 °C, 60 °C and 66 °C. After 30 minutes substrate (EnzChek® Ultra Xylanase Assay Kit) was added and the fluorescence was measured for 150 minutes in a platereader at 37 °C. The results indicate a reduction of activity at temperatures higher than 50 °C and complete loss of function for temperatures higher than 60 °C.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 153
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 557
    Illegal SapI.rc site found at 611