Difference between revisions of "Part:BBa K1396000:Experience"
(→User Reviews) |
(→User Reviews) |
||
| Line 13: | Line 13: | ||
|width='10%'| | |width='10%'| | ||
<partinfo>BBa_K1396000 AddReview number</partinfo> | <partinfo>BBa_K1396000 AddReview number</partinfo> | ||
| − | <I> | + | <I>ahleyva65</I> |
|width='60%' valign='top'| | |width='60%' valign='top'| | ||
| + | |||
Team 2014 Yale iGEM ordered this part and attempted to express it within a standard pZE21 plasmid with a pLtetO promoter. Expression may have been successful, but part is extremely toxic and stalls cell growth significantly. In addition, the part is meant for expression in recoded Amberless E. Coli with either a Tyrosine Supressor or L-DOPA Orthogonal Translation System. In the case of L-DOPA the device must expressed in Minimal Media with DTT added one hour before L-DOPA and inducer, although the growth of the E. Coli in Minimal Media was insubstantial for characterization. | Team 2014 Yale iGEM ordered this part and attempted to express it within a standard pZE21 plasmid with a pLtetO promoter. Expression may have been successful, but part is extremely toxic and stalls cell growth significantly. In addition, the part is meant for expression in recoded Amberless E. Coli with either a Tyrosine Supressor or L-DOPA Orthogonal Translation System. In the case of L-DOPA the device must expressed in Minimal Media with DTT added one hour before L-DOPA and inducer, although the growth of the E. Coli in Minimal Media was insubstantial for characterization. | ||
Revision as of 03:55, 1 November 2014
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K1396000
User Reviews
UNIQ165189f8bd9eab68-partinfo-00000000-QINU UNIQ165189f8bd9eab68-partinfo-00000001-QINU