Difference between revisions of "Part:BBa K1440001"
Eric423627 (Talk | contribs) |
|||
Line 1: | Line 1: | ||
− | |||
__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K1440001 short</partinfo> | <partinfo>BBa_K1440001 short</partinfo> | ||
− | + | This biobrick is used to express Cre-ERTII protein. Cre when is binding the ERT2 sequence together, it will be not activated until tamoxifen inducing it. | |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Latest revision as of 17:48, 31 October 2014
Cre protein with ERT2 sequence
This biobrick is used to express Cre-ERTII protein. Cre when is binding the ERT2 sequence together, it will be not activated until tamoxifen inducing it.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 279
Illegal XbaI site found at 2259
Illegal PstI site found at 463
Illegal PstI site found at 634
Illegal PstI site found at 2443
Illegal PstI site found at 2614 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 463
Illegal PstI site found at 634
Illegal PstI site found at 2443
Illegal PstI site found at 2614 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 413
Illegal BglII site found at 2393
Illegal BamHI site found at 1286
Illegal XhoI site found at 1960 - 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 279
Illegal XbaI site found at 2259
Illegal PstI site found at 463
Illegal PstI site found at 634
Illegal PstI site found at 2443
Illegal PstI site found at 2614 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 279
Illegal XbaI site found at 2259
Illegal PstI site found at 463
Illegal PstI site found at 634
Illegal PstI site found at 2443
Illegal PstI site found at 2614
Illegal AgeI site found at 972 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 313
Illegal BsaI site found at 2293