Difference between revisions of "Part:BBa K1413044:Experience"
(→Applications of BBa_K1413044) |
(→User Reviews) |
||
| Line 30: | Line 30: | ||
|width='60%' valign='top'| | |width='60%' valign='top'| | ||
Enter the review inofrmation here. | Enter the review inofrmation here. | ||
| − | <img src="https://static.igem.org/mediawiki/2014/d/df/Tranposons.png" | + | <img src="https://static.igem.org/mediawiki/2014/d/df/Tranposons.png"/> |
|}; | |}; | ||
<!-- End of the user review template --> | <!-- End of the user review template --> | ||
<!-- DON'T DELETE --><partinfo>BBa_K1413044 EndReviews</partinfo> | <!-- DON'T DELETE --><partinfo>BBa_K1413044 EndReviews</partinfo> | ||
Revision as of 16:00, 31 October 2014
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K1413044
To replicate this new plasmid we can used E. coli. To separate the pSB1C3 and new pNK2 we must used BglII. After that the disgest product must migrate in the electrophoresis gel.The band to 2,6 kb , which is the biggest correspond to transposon plasmid. The transposon plasmid can be extract in the gel. The T4 ligase is used to circularize the plasmid.
All the biobrick can introduce between the Is10 with biobrick enzyme. Also this biobrick can be integrate in Pseudovibrio denitrificans the genome with electro transformation. The voltage used is 2000V and the incubation time after the electroporation is to 3h.
Picture of an electrophorese gel samples come from the PCR which amplify the kanamycineR cassette. All clones have the insertion of the transposon. The Control, Pseudovibrio denitrificans, do not the insertion as expected.
User Reviews
UNIQ02c2a9bedff1d1d1-partinfo-00000001-QINU UNIQ02c2a9bedff1d1d1-partinfo-00000002-QINU