Difference between revisions of "Part:BBa K1523021"
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nahR, encoding a LysR-type transcriptional regulator, is highly conserved among naphthalene-degrading bacteria isolated from a coal tar waste-contaminated site and in extracted community DNA. It contains a kind of HTH LysR DNA-binding domain.
 
nahR, encoding a LysR-type transcriptional regulator, is highly conserved among naphthalene-degrading bacteria isolated from a coal tar waste-contaminated site and in extracted community DNA. It contains a kind of HTH LysR DNA-binding domain.
  
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NAH7 plasmid specify catabolism of naphthalene and salicylate under positive regulation by gene nahR. A 1.75-kb fragment (PstI-HindIII) cloned into the pCP13 derivative of vector RK2 complemented in trans five nahR mutations. The fragment sequence contained a 1,122-base-pair open reading frame with a predicted sequence of 374 residues that was rich in basic amino acids with regions similar to known DNA-binding proteins. Clones from the nahR gene region were expressed in mexicells. Plasmid pY1923, carrying the 1.75-kb PstI-HindIII fragment, expressed a protein of Mr ca. 35,000 which bound to the upstream region of gene nahR in a gel electrophoresis DNA-binding assay. Other clones expressed proteins of currently unknown function; pY1311, with the 1.1-kb HindIII fragment, produced a polypeptide with an Mr of 23,000, and pY1812, with the 1.2-kb PstI-SphI fragment, produced a polypeptide (Mr 41,000) which appeared to be a fused nahR-lacZ product.
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NAH7 plasmid specify catabolism of naphthalene and salicylate under positive regulation by gene nahR. Clones from the nahR gene region were expressed in mexicells by You in 1988. Plasmid pY1923, carrying the 1.75-kb PstI-HindIII fragment, expressed a protein of Mr ca. 35,000 which bound to the upstream region of gene nahR.  
  
 
Operons repressed by nahR can be regulated positively by nahR when '''salicylate''' appears.
 
Operons repressed by nahR can be regulated positively by nahR when '''salicylate''' appears.
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[[File:File:Vector_Nah.jpg]]
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The Vector NAH7. Both nah operon and sal operon are regulated by nahR.
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Reference
  
  

Revision as of 15:49, 31 October 2014

nahR coding sequence,encoding a LysR-type transcriptional regulator

nahR, encoding a LysR-type transcriptional regulator, is highly conserved among naphthalene-degrading bacteria isolated from a coal tar waste-contaminated site and in extracted community DNA. It contains a kind of HTH LysR DNA-binding domain.

NAH7 plasmid specify catabolism of naphthalene and salicylate under positive regulation by gene nahR. Clones from the nahR gene region were expressed in mexicells by You in 1988. Plasmid pY1923, carrying the 1.75-kb PstI-HindIII fragment, expressed a protein of Mr ca. 35,000 which bound to the upstream region of gene nahR.

Operons repressed by nahR can be regulated positively by nahR when salicylate appears.


File:File:Vector Nah.jpg

The Vector NAH7. Both nah operon and sal operon are regulated by nahR.


Reference


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 57
    Illegal PstI site found at 250
    Illegal PstI site found at 469
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 57
    Illegal PstI site found at 250
    Illegal PstI site found at 469
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 323
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 57
    Illegal PstI site found at 250
    Illegal PstI site found at 469
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 57
    Illegal PstI site found at 250
    Illegal PstI site found at 469
    Illegal NgoMIV site found at 491
    Illegal NgoMIV site found at 646
  • 1000
    COMPATIBLE WITH RFC[1000]