Difference between revisions of "Part:BBa K1347003"
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The 2014 Nevada iGEM team cloned this part into a yeast integrating vector and observed its expression and functionality. | The 2014 Nevada iGEM team cloned this part into a yeast integrating vector and observed its expression and functionality. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 02:46, 31 October 2014
COI1
COI1 - COI1 is an E3 ligase that interacts with Jaz proteins (leading to their subsequent ubiquitination and degradation) after coronatin is introduced to the system. It is a system found in plants that is analogous to the auxin-TIR1-AID system studied by the Evry 2012 team.
The 2014 Nevada iGEM team cloned this part into a yeast integrating vector and observed its expression and functionality.
Usage and Biology
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1260
Illegal BglII site found at 1590
Illegal BamHI site found at 6
Illegal XhoI site found at 967 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 826