Difference between revisions of "Part:BBa K1391132:Design"

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<partinfo>BBa_K1391132 short</partinfo>
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<partinfo>BBa_K1391030 short</partinfo>
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<partinfo>BBa_K1391030 SequenceAndFeatures</partinfo>
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<p> This part was created using scarless golden gate assembly. This basic part is flanked by L1 and L2 sites and can be easily cloned into an entry vector using an LR reaction. A promoter can be easily inserted in front of this part in a one pot LR reaction with a promoter flanked by L4 and R1 sites cloned into a backbone that has a negative selection marker between R4 and R2 sites. This part adheres to RFC 65 for recombination based cloning of mammalian parts. </p>
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<partinfo>BBa_K1391132 SequenceAndFeatures</partinfo>
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<img src="https://static.igem.org/mediawiki/parts/2/2b/BACE1_Dosnregulation_Circuit.png" width=100% align=center></br>
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===Design Notes===
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This part was created using a single pot LR reaction. The promoter is flanked by B4 and P1 sites and the gene is flanked by P1 and B2 sites. Either of these can be extracted using a BP reaction. This part adheres to RFC 65 for recombination based cloning of mammalian parts.
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===Source===
 
===Source===

Revision as of 21:51, 1 November 2014

pENTR_miRNAG1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 502
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 502
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 502
    Illegal XhoI site found at 398
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 502
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 502
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1024
    Illegal SapI.rc site found at 16


This part was created using scarless golden gate assembly. This basic part is flanked by L1 and L2 sites and can be easily cloned into an entry vector using an LR reaction. A promoter can be easily inserted in front of this part in a one pot LR reaction with a promoter flanked by L4 and R1 sites cloned into a backbone that has a negative selection marker between R4 and R2 sites. This part adheres to RFC 65 for recombination based cloning of mammalian parts.



Source

Artificial

References