Difference between revisions of "Part:BBa J45992:Experience"
| Line 6: | Line 6: | ||
J45992 was characterized by measuring flourescence intensity of the in a plate reader. The characterization device was <bbpart>J45995</bbpart>. In figure A, the absorbance, which is proportional to cell density, is plotted against fluorescence, or roughly the number of GFP molecules present in the culture. It is seen in the graph that J45995 (J45992.E0840) exhibits a much higher level of fluorescence during stationary phase (Absorbance>0.5, see figure B) than during exponential phase (yellow line). Additionally, J45992 was attached to an inverter (<bbpart>Q04401</bbpart>) and the signal was effectively turned off at stationary phase (green line). | J45992 was characterized by measuring flourescence intensity of the in a plate reader. The characterization device was <bbpart>J45995</bbpart>. In figure A, the absorbance, which is proportional to cell density, is plotted against fluorescence, or roughly the number of GFP molecules present in the culture. It is seen in the graph that J45995 (J45992.E0840) exhibits a much higher level of fluorescence during stationary phase (Absorbance>0.5, see figure B) than during exponential phase (yellow line). Additionally, J45992 was attached to an inverter (<bbpart>Q04401</bbpart>) and the signal was effectively turned off at stationary phase (green line). | ||
| − | [[Image:BBa J45992-3characterization.png|left|thumb|600px|<font size=3>Figure A - J45992 chracterization (yellow) | + | [[Image:BBa J45992-3characterization.png|left|thumb|600px|<font size=3>Figure A - J45992 chracterization (yellow), with signal inverted (green), with control constitutive promoter (grey), and with no fluorescent protein coding region (purple)</font>]][[Image:osmYgraph.jpg|right|thumb|600px|<font size=3>Figure B - Graph shows that cell reach stationary phase at absorbance ~0.5</font>]] |
===User Reviews=== | ===User Reviews=== | ||
Revision as of 04:10, 31 October 2006
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_J45992
J45992 was characterized by measuring flourescence intensity of the in a plate reader. The characterization device was J45995. In figure A, the absorbance, which is proportional to cell density, is plotted against fluorescence, or roughly the number of GFP molecules present in the culture. It is seen in the graph that J45995 (J45992.E0840) exhibits a much higher level of fluorescence during stationary phase (Absorbance>0.5, see figure B) than during exponential phase (yellow line). Additionally, J45992 was attached to an inverter (Q04401) and the signal was effectively turned off at stationary phase (green line).
User Reviews
UNIQaeed828dcf8285ad-partinfo-00000002-QINU UNIQaeed828dcf8285ad-partinfo-00000003-QINU