Difference between revisions of "Part:BBa J45992"
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This promoter is active in stationary phase and under high osmotic pressure conditions. See experience section for device characterization using <bbpart>J45995</bbpart> (promoter attached to GFP). | This promoter is active in stationary phase and under high osmotic pressure conditions. See experience section for device characterization using <bbpart>J45995</bbpart> (promoter attached to GFP). | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
This promoter is significantly more active in sationary phase. Schellhorn, et. al were able to connect the promoter to the LacZ gene in strains grown in rich media. They were able to show that the beta-Galactosidase activity in stationary phase was 408.8 Miller Units, while the activity was only 8.6 Miller Units in exponential phase. This preferential activation of the osmY promoter is the result of a complex network of inhibitors and activators which act on the promoter during various stages of the cell cycle. The promoter is specifically inhibited by H-NS, Lrp, cAMP-CRP, and IHF, while it is specifically activated by the sigma s factor, which is stronly activated by rpos in stationary phase or under starvation conditions (Neidhart 1502-1504). | This promoter is significantly more active in sationary phase. Schellhorn, et. al were able to connect the promoter to the LacZ gene in strains grown in rich media. They were able to show that the beta-Galactosidase activity in stationary phase was 408.8 Miller Units, while the activity was only 8.6 Miller Units in exponential phase. This preferential activation of the osmY promoter is the result of a complex network of inhibitors and activators which act on the promoter during various stages of the cell cycle. The promoter is specifically inhibited by H-NS, Lrp, cAMP-CRP, and IHF, while it is specifically activated by the sigma s factor, which is stronly activated by rpos in stationary phase or under starvation conditions (Neidhart 1502-1504). | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_J45992 SequenceAndFeatures</partinfo> | <partinfo>BBa_J45992 SequenceAndFeatures</partinfo> | ||
Revision as of 03:22, 31 October 2006
Full-length stationary phase osmY promoter
This promoter is active in stationary phase and under high osmotic pressure conditions. See experience section for device characterization using J45995 (promoter attached to GFP).
Usage and Biology
This promoter is significantly more active in sationary phase. Schellhorn, et. al were able to connect the promoter to the LacZ gene in strains grown in rich media. They were able to show that the beta-Galactosidase activity in stationary phase was 408.8 Miller Units, while the activity was only 8.6 Miller Units in exponential phase. This preferential activation of the osmY promoter is the result of a complex network of inhibitors and activators which act on the promoter during various stages of the cell cycle. The promoter is specifically inhibited by H-NS, Lrp, cAMP-CRP, and IHF, while it is specifically activated by the sigma s factor, which is stronly activated by rpos in stationary phase or under starvation conditions (Neidhart 1502-1504).
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]