Difference between revisions of "Part:BBa C0012"

Revision as of 01:49, 30 April 2006

lacI repressor from E. coli (+LVA)

Coding region for the LacI protein with an LVA degradation tail and without an RBS. LacI binds to the pLac regulator BBa_R0010 and PLlac01 hybrid regulator BBa_R0011 and inhibits transcription. IPTG (Isopropylthiogalactoside) binds to LacI and inhibits its operation, therefore promoting transcription.

A rapid degredation tail (LVA) has been added to improve the High to Low performance of this part.

Usage and Biology

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Sequence and Features

Barcodes are discontinued, but one was appended to the sequence of this part. Composite parts using this part will include the barcode. More ...

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1147
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Functional Parameters

direction	Forward
function	Rep
ligands	allolactose, IPTG
protein	LacI
swisspro	P03023
tag	LVA

@@ Line 3: / Line 3: @@
 <partinfo>BBa_C0012 short</partinfo>
-Coding region for the LacI protein with an LVA degradation tail and without      an RBS. LacI binds to the pLac regulator <bbpart>BBa_R0010</bbpart> and PLlac01 hybrid regulator <bbpart>BBa_R0011</bbpart> and inhibits transcription. IPTG (Isopropylthiogalactoside) binds      to LacI and inhibits its operation, therefore promoting transcription.</P>   <P>A rapid degredation tail (LVA) has been added to improve the High to Low      performance of this part.</P>
+Coding region for the LacI protein with an LVA degradation tail and without      an RBS. LacI binds to the pLac regulator <bbpart>BBa_R0010</bbpart> and PLlac01 hybrid regulator <bbpart>BBa_R0011</bbpart> and inhibits transcription. IPTG (Isopropylthiogalactoside) binds      to LacI and inhibits its operation, therefore promoting transcription.
+A rapid degredation tail (LVA) has been added to improve the High to Low      performance of this part.
 ===Usage and Biology===