Difference between revisions of "Part:BBa K1321352:Design"

(Design Notes)
(Source)
Line 13: Line 13:
 
===Source===
 
===Source===
  
asdf
+
Our NiBP came from BBa_K1151001.
 +
 
 +
The T7 vector was made by inverse PCR of BBa_I746909, and ligated. Please see our BBa_ K1321338 for more information on this part.
  
 
===References===
 
===References===

Revision as of 19:57, 24 October 2014


NiBP fused to CBDcex driven by T7


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 53
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 232


Design Notes

The fusion protein was cloned together using the NgoMIV and AgeI sites.

The promotor was cloned onto the protein via the XbaI/SpeI sites.

Source

Our NiBP came from BBa_K1151001.

The T7 vector was made by inverse PCR of BBa_I746909, and ligated. Please see our BBa_ K1321338 for more information on this part.

References