Difference between revisions of "Part:BBa K1321345:Design"
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===Source=== | ===Source=== | ||
| − | + | Our NiBP came from BBa_K1151001. | |
| − | + | dCBD with its linker were synthesized from Geneart and cloned into the psB1C3 backbone. | |
===References=== | ===References=== | ||
Latest revision as of 18:52, 24 October 2014
NiBP fused to dCBD with linker in RFC 25
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 174
Design Notes
The fusion protein was cloned together using the NgoMIV and AgeI sites.
Source
Our NiBP came from BBa_K1151001. dCBD with its linker were synthesized from Geneart and cloned into the psB1C3 backbone.