Difference between revisions of "Part:BBa K1321344:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | Our NiBP came from BBa_K1151001. CBDcex was requested from the registry, as it was submitted by the Freiburg team as BBa_K863101. | |
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===Source=== | ===Source=== | ||
Revision as of 18:46, 24 October 2014
NiBP fused to CBDcex in RFC 25
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 174
Design Notes
Our NiBP came from BBa_K1151001. CBDcex was requested from the registry, as it was submitted by the Freiburg team as BBa_K863101.
Source
The fusion protein was cloned together using the NgoMIV and AgeI sites. The promotor was cloned onto the protein via the XbaI/SpeI sites.