Difference between revisions of "Part:BBa K1371026:Design"
 
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===Design Notes===
 
===Design Notes===
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The design of docking domains in our experiment. At each set of docking domain, the top was connected with ACP that its upstream joined in the RFC 23 and its downstream joined in the RFC 10. The following came close to KS that the upstream joined in the RFC 10 and the downstream joined in the RFC 23. The following has no linker generally.
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<div class="center">
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[[File:DD-figb3 .jpg]]
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</div>
 
===Source===
 
===Source===
  

Latest revision as of 18:47, 24 October 2014

Docking domain 6 pre


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 126
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The design of docking domains in our experiment. At each set of docking domain, the top was connected with ACP that its upstream joined in the RFC 23 and its downstream joined in the RFC 10. The following came close to KS that the upstream joined in the RFC 10 and the downstream joined in the RFC 23. The following has no linker generally.

DD-figb3 .jpg

Source

Saccharopolyspora erythraea

References

[1]Broadhurst RW, Nietlispach D, Wheatcroft MP, Leadlay PF, Weissman KJ. The structure of docking domains in modular polyketide synthases [J]. Chem Biol, 2003, 10: 723–731.

[2] Worthington, A. S., Hur, G. H., Meier, J. L., Cheng, Q., Moore, B. S.and Burkart, M. D. . Probing the compatibility of type II ketosynthase-carrier protein partners [J]. ChemBioChem, 2008, 9: 2096 –2103

[3]onia JB, Todd WG, Frank EB Kevin AR, Janet LS, and David HS. Structural Basis for Binding Specificity between Subclasses of Modular Polyketide Synthase Docking Domains [J]. ACS Chem Biol, 2009, Vol.4 No.1

[4]Jonathan RW, Sarah SS, Douglas AH, William CB, William HG,David HS and Janet LS. Cyanobacterial Polyketide Synthase Docking Domains:A Tool for Engineering Natural Product Biosynthesis [J]. Chem Biol, 2013, 20:1340–1351.

[5]Tang, Y., Kim, C.-Y., Mathews, I. I., Cane, D. E. and Khosla, C. . The 2.7-angstrom crystal structure of a 194-kDa homodimeric fragment of the 6-deoxyerythronolide B synthase [J]. Proc. Natl. Acad. Sci. U.S.A. 2006, 103, 11124 -11129.