Difference between revisions of "Part:BBa B0013"
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===Usage and Biology=== | ===Usage and Biology=== | ||
− | <img src="'''Include File TE.gif'''" width="413" height="246"> | + | <img src="'''Include File TE.gif'''" width="413" height="246"> |
+ | |||
+ | Above is a picture of the wildtype terminator. This terminator has been altered, see design notes. | ||
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> |
Revision as of 01:49, 30 April 2006
TE from coliphage T7 (+/-)
Strong transcriptional terminator consisting of a 20 bp stem-loop that has been engineered to be bidirectional.
Usage and Biology
<img src="Include File TE.gif" width="413" height="246">
Above is a picture of the wildtype terminator. This terminator has been altered, see design notes.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Functional Parameters
biology | T7TE (+/-) |
direction | Forward |
forward_efficiency | 0.6[CC] |
reversed_version | 591 |
reverse_efficiency | -1.06[CC] |