Difference between revisions of "Part:BBa I14017:Experience"
(→Modeling crosstalk) |
(→Modeling crosstalk) |
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[[File:ETHZ_HillEq.png|center|200px]] | [[File:ETHZ_HillEq.png|center|200px]] | ||
<p>The fitting of the graphs was performed using the following equation :<br><br> | <p>The fitting of the graphs was performed using the following equation :<br><br> | ||
− | rFluo = the relative fluorescence (absolute measured fluorescence value over OD)[ | + | rFluo = the relative fluorescence (absolute measured fluorescence value over OD)[a.u.]<br> |
− | a = basal expression rate [ | + | a = basal expression rate [a.u.](“leakiness”)<br> |
− | b = maximum expression rate [ | + | b = maximum expression rate [a.u.]("full induction")<br> |
n = Hill coefficient (“cooperativity”)<br> | n = Hill coefficient (“cooperativity”)<br> | ||
K<sub>m</sub> = Half-maximal effective concentration (“sensitivity”)<br> | K<sub>m</sub> = Half-maximal effective concentration (“sensitivity”)<br> | ||
Line 49: | Line 49: | ||
|+ Parameters of HillFunction for crosstalk with Prhl (with 95% confidence bounds) | |+ Parameters of HillFunction for crosstalk with Prhl (with 95% confidence bounds) | ||
! | ! | ||
− | ! C4-HSL | + | ! [[AHL|C4-HSL]] |
− | ! 3OC6-HSL | + | ! [[3OC6HSL|3OC6-HSL]] |
− | ! 3OC12-HSL | + | ! [[AHL|3OC12-HSL]] |
|- | |- | ||
− | ! RhlR | + | ! [https://parts.igem.org/Part:BBa_C0171 RhlR] |
− | | a = 178.4 (174.9, 182)<br> n = 1.053 (0.9489, 1.157)<br> Km = 1969 (1625, 2313)<br>b = 1736 (1629, 1842)<br> | + | | a = 178.4 (174.9, 182) [a.u.]<br> n = 1.053 (0.9489, 1.157)<br> Km = 1969 (1625, 2313) [nM]<br>b = 1736 (1629, 1842) [a.u.]<br> |
− | | a = 169.1 (155.2, 182.9) <br> n = 0.507 (0.2303, 0.7837) <br> Km = 1.08e8(0, 2.681e10) <br> b = 9.708e4 (0, 1.192e7)<br> | + | | a = 169.1 (155.2, 182.9) [a.u.]<br> n = 0.507 (0.2303, 0.7837) <br> Km = 1.08e8(0, 2.681e10) [nM]<br> b = 9.708e4 (0, 1.192e7) [a.u.]<br> |
− | | a = 162.8 (150.4, 175.1)<br> n = 0.404 (0, 0.998)<br> Km = 9.627e8 (0, 7.824e11)<br>b = 2.537e4 (0, 8.109e6)<br> | + | | a = 162.8 (150.4, 175.1) [a.u.]<br> n = 0.404 (0, 0.998)<br> Km = 9.627e8 (0, 7.824e11) [nM]<br>b = 2.537e4 (0, 8.109e6) [a.u.]<br> |
|- | |- | ||
− | ! LuxR | + | ! [https://parts.igem.org/Part:BBa_C0062 LuxR] |
| No crosstalk | | No crosstalk | ||
| No crosstalk | | No crosstalk | ||
| No crosstalk | | No crosstalk | ||
|- | |- | ||
− | ! LasR | + | ! [https://parts.igem.org/Part:BBa_C0179 LasR] |
| No crosstalk | | No crosstalk | ||
| No crosstalk | | No crosstalk | ||
− | | a = 149.3 (140.6, 158.1) <br>n = 1.366 (0.808, 1.923) <br> Km = 1.674 (1.259, 2.09)<br> b = 628.9 (599, 658.7)<br> | + | | a = 149.3 (140.6, 158.1) [a.u.]<br>n = 1.366 (0.808, 1.923) <br> Km = 1.674 (1.259, 2.09) [nM]<br> b = 628.9 (599, 658.7) [a.u.]<br> |
|} | |} | ||
Revision as of 12:01, 24 October 2014
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Applications of BBa_I14017
User Reviews
UNIQ0ea184695dcdbc34-partinfo-00000000-QINU
••••
ETH Zurich 2014 |
Characterization of two-order crosstalk on the promoterBackground informationSystem consideredModeling crosstalkEach experimental data set was fitted to an Hill function using the Least Absolute Residual method. The fitting of the graphs was performed using the following equation :
First-order crosstalkFirst Level crosstalk: RhlR binds to different HSL and activates the promoterSecond Level crosstalk: other regulatory proteins, like LuxR, bind to their natural HSL substrate and activates the promoterSecond order crosstalk: Combination of both cross-talk levelsOther regulatory proteins, like LuxR, bind to different HSL and activates the promoter. Results
|
Antiquity |
This review comes from the old result system and indicates that this part did not work in some test. |
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