Difference between revisions of "Part:BBa K1499201"
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===Usage and Biology=== | ===Usage and Biology=== | ||
''Deinococcus radiodurans'' is a highly radiation-resistant bacterium that can survive after up to 12,000 Gy (absorbed radiation dose, Gray) of radiation. The unique genes that allow it to better handle radiation exposure may provide ways to make ''E. coli'' and other organisms more resistant for applications of synthetic biology in tough environmental conditions, like space. ''D. radiodurans'' is known to survive both ionizing and UV radiation. | ''Deinococcus radiodurans'' is a highly radiation-resistant bacterium that can survive after up to 12,000 Gy (absorbed radiation dose, Gray) of radiation. The unique genes that allow it to better handle radiation exposure may provide ways to make ''E. coli'' and other organisms more resistant for applications of synthetic biology in tough environmental conditions, like space. ''D. radiodurans'' is known to survive both ionizing and UV radiation. | ||
Revision as of 00:40, 24 October 2014
Deinococcus radiodurans uracil-DNA glycosylase 1
TODO
Usage and Biology
Deinococcus radiodurans is a highly radiation-resistant bacterium that can survive after up to 12,000 Gy (absorbed radiation dose, Gray) of radiation. The unique genes that allow it to better handle radiation exposure may provide ways to make E. coli and other organisms more resistant for applications of synthetic biology in tough environmental conditions, like space. D. radiodurans is known to survive both ionizing and UV radiation.
One gene that contributes to this phenotype is a family 1 uracil-DNA glycosylase (UDG), which catalyzes the excision and repair of cytosine->uracil mutations that occur following UV exposure [http://www.ncbi.nlm.nih.gov/pubmed/11807060 (1)][http://www.ncbi.nlm.nih.gov/pubmed/10946227 (2)]. The enzyme can remove uracil from U:G and U:A pairs in both double-stranded and single-stranded DNA. Out of the four families of UDGs, family 1 UDGs are found in E. coli, humans, and DNA viruses [http://www.ncbi.nlm.nih.gov/pubmed/11807060 (1)].
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 720
- 1000COMPATIBLE WITH RFC[1000]