Difference between revisions of "Part:BBa J37016:Design"
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with LuxI under the control of Lux pR *LuxR and GFP are under the control of the promoter luxpR, which is activated by the heterodimer AHL/LuxR | with LuxI under the control of Lux pR *LuxR and GFP are under the control of the promoter luxpR, which is activated by the heterodimer AHL/LuxR | ||
*The part has been ligated from the following previous parts: <bbpart>BBa_J37019</bbpart>, | *The part has been ligated from the following previous parts: <bbpart>BBa_J37019</bbpart>, | ||
| − | <bbpart>J37025</bbpart> | + | <bbpart>J37025</bbpart>*Expression of GFP downstream of the promoter serves as an indicator of the production of LuxR. |
| − | *Expression of GFP downstream of the promoter serves as an indicator of the production of LuxR. | + | |
| − | + | ||
*More details about design and motivation behind the part can be found [http://openwetware.org/wiki/IGEM:IMPERIAL/2006/project/Oscillator/project_browser/Test_Sensing_Predator_Construct/Design here]. | *More details about design and motivation behind the part can be found [http://openwetware.org/wiki/IGEM:IMPERIAL/2006/project/Oscillator/project_browser/Test_Sensing_Predator_Construct/Design here]. | ||
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===Source=== | ===Source=== | ||
Latest revision as of 15:14, 30 October 2006
Predator Molecule Generator Sensing Test (GFP output)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1532
Design Notes
- The part is designed to produce both LuxR and GFP in response to different AHL concentrations.
with LuxI under the control of Lux pR *LuxR and GFP are under the control of the promoter luxpR, which is activated by the heterodimer AHL/LuxR
- The part has been ligated from the following previous parts: BBa_J37019,
J37025*Expression of GFP downstream of the promoter serves as an indicator of the production of LuxR.
- More details about design and motivation behind the part can be found [http://openwetware.org/wiki/IGEM:IMPERIAL/2006/project/Oscillator/project_browser/Test_Sensing_Predator_Construct/Design here].
Source
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