Difference between revisions of "Part:BBa M36921:Design"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_M36921 short</partinfo>
 
<partinfo>BBa_M36921 short</partinfo>
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===Design Notes===
 
===Design Notes===
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We had to enter silent mutations to change the DNA sequence without affecting the amino acid sequence because there were palindromes greater than 10 bp in every basic part.
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We had to enter silent mutations to change the DNA sequence of our gene without affecting the amino acid sequence because there were palindromes greater than 10 bp.
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+
  
  
 
===Source===
 
===Source===
  
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The genomic sequence comes from parts of an E. Coli protein (ZntR).
+
The genomic sequences come from various parts of an E. Coli protein (ZntR).
  
 
===References===
 
===References===

Revision as of 23:43, 23 October 2014

Zinc Sensor


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 194
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal PstI site found at 194
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 190
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 194
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 194
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We had to enter silent mutations to change the DNA sequence of our gene without affecting the amino acid sequence because there were palindromes greater than 10 bp.


Source

The genomic sequences come from various parts of an E. Coli protein (ZntR).

References