Difference between revisions of "Part:BBa M36921"

Revision as of 23:46, 23 October 2014

Zinc Sensor

This composite part contains a constitutive promoter, ribosome-binding-site, a gene for ZntR, an E. Coli terminator, and a promoter that is ZntR regulated. In the absence of Zinc, the ZntR protein binds to the ZntR promoter binding site. In the presence of zinc, ZntR undergoes a conformation, does not attach to the binding site, and allows for transcription of our actuator. Essentially, this part acts as a sensor for the presence of zinc.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 194
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal PstI site found at 194
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 190
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 194
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 194
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_M36921 short</partinfo>
-This composite part contains a promoter and ribosome-binding-site, a gene for ZntR, a terminator, and a promoter for ZntA. In the absence of Zinc, the ZntR protein binds to the ZntA binding site. In the presence of zinc, ZntR undergoes a conformation, does not attach to the binding site, and allows for transcription of ZntA. Essentially, this part acts as a sensor for the presence of zinc.
+This composite part contains a constitutive promoter, ribosome-binding-site, a gene for ZntR, an E. Coli terminator, and a promoter that is ZntR regulated. In the absence of Zinc, the ZntR protein binds to the ZntR promoter binding site. In the presence of zinc, ZntR undergoes a conformation, does not attach to the binding site, and allows for transcription of our actuator. Essentially, this part acts as a sensor for the presence of zinc.
 <!-- Add more about the biology of this part here