Difference between revisions of "Part:BBa K1431832"
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We've constructed a series of plasmids which are all in the same pattern: a strong/weak promoter, a strong/weak RBS and a chromoprotein. We want to monitor the speed of expression of these plasmids in normal incubation conditions (like 37℃ overnight for LB agar plate and 37℃ 180rpm for LB broth). The expression speed and strength of the constructed biobricks will be carefully monitored and gave others a relative scale for using chromoprotein as a reporter gene. | We've constructed a series of plasmids which are all in the same pattern: a strong/weak promoter, a strong/weak RBS and a chromoprotein. We want to monitor the speed of expression of these plasmids in normal incubation conditions (like 37℃ overnight for LB agar plate and 37℃ 180rpm for LB broth). The expression speed and strength of the constructed biobricks will be carefully monitored and gave others a relative scale for using chromoprotein as a reporter gene. | ||
| + | '''For detailed characterization data, see the experience page.''' | ||
===Selective Figures=== | ===Selective Figures=== | ||
<center>https://static.igem.org/mediawiki/parts/a/a1/SUSTC-Shenzhen-Project-P41016-112140.jpg</center> | <center>https://static.igem.org/mediawiki/parts/a/a1/SUSTC-Shenzhen-Project-P41016-112140.jpg</center> | ||
Revision as of 13:08, 27 October 2014
amilCP, blue chromoprotein reporter system (Weak Promoter, Strong RBS)
Team Uppsala 2012 chromoprotein attracts many interests because its more convenient than fluorescent protein to be use as a reporter. However, few characterized data can be found for these proteins. SUSTC-Shenzhen this year want to tackle with theses problems.
We've constructed a series of plasmids which are all in the same pattern: a strong/weak promoter, a strong/weak RBS and a chromoprotein. We want to monitor the speed of expression of these plasmids in normal incubation conditions (like 37℃ overnight for LB agar plate and 37℃ 180rpm for LB broth). The expression speed and strength of the constructed biobricks will be carefully monitored and gave others a relative scale for using chromoprotein as a reporter gene.
For detailed characterization data, see the experience page.
Selective Figures
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]