Difference between revisions of "Part:BBa K1321114:Design"
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===Design Notes=== | ===Design Notes=== | ||
CBD4+PhytoC with LacI | CBD4+PhytoC with LacI | ||
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===Source=== | ===Source=== |
Revision as of 20:37, 21 October 2014
CBDclos fused to phytochelatin (PC) EC20 with LacI promoter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 230
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
CBD4+PhytoC with LacI
Source
Phytochelatin was synthesized from Geneart and cloned into the psB1C3 backbone. CBDclos was requested from the registry, as it was submitted by the Freiburg team as BBa_K863111. The LacI part came from BBa_J04500.