Difference between revisions of "Part:BBa K1321110:Design"
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===Source=== | ===Source=== | ||
| − | + | Phytochelatin and dCBD with its linker were synthesized from Geneart and cloned into the psB1C3 backbone. | |
The LacI part was made by inverse PCR of BBa_J04500, and ligated. | The LacI part was made by inverse PCR of BBa_J04500, and ligated. | ||
===References=== | ===References=== | ||
Revision as of 20:17, 21 October 2014
Phytochelatin (PC) EC20 fused to linker-dCBD with LacI
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 230
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Once the individual parts were confirmed in Freiburg format, we cloned them using the AgeI and NgoMIV site, creating a scar that also functions as a small linker. The LacI vector was fused to the protein fusion using the Xba and SpeI site.
Source
Phytochelatin and dCBD with its linker were synthesized from Geneart and cloned into the psB1C3 backbone. The LacI part was made by inverse PCR of BBa_J04500, and ligated.