Difference between revisions of "Part:BBa K1321104:Design"
(→Design Notes) |
(→Source) |
||
| Line 12: | Line 12: | ||
===Source=== | ===Source=== | ||
| − | + | Phytochelatin was synthesized from Geneart and cloned into the psB1C3 backbone. CBDclos was requested from the registry, as it was submitted by the Freiburg team as BBa_K863111. | |
| + | The T7 vector was made by inverse PCR of BBa_I746909, and ligated. Please see our BBa_ K1321338 for more information on this part. | ||
===References=== | ===References=== | ||
Latest revision as of 19:53, 21 October 2014
CBDclos fused to Phytochelatin (PC) EC20 with T7 promoter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 53
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Codon optimised for E.coli. Once the individual parts were confirmed in Freiburg format, we cloned them using the AgeI and NgoMIV site, creating a scar that also functions as a small linker. The T7 vector was fused to the protein fusion using the Xba and SpeI site.
Source
Phytochelatin was synthesized from Geneart and cloned into the psB1C3 backbone. CBDclos was requested from the registry, as it was submitted by the Freiburg team as BBa_K863111. The T7 vector was made by inverse PCR of BBa_I746909, and ligated. Please see our BBa_ K1321338 for more information on this part.