Difference between revisions of "Part:BBa K1321097:Design"
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===Source=== | ===Source=== | ||
| − | + | Phytochelatin and CBDcenA were synthesized from Geneart and cloned into the psB1C3 backbone. | |
===References=== | ===References=== | ||
Revision as of 19:27, 21 October 2014
CBDcenA-linker fused to Phytochelatin (PC) EC20
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 372
Design Notes
Codon optimised for E.coli. Once the individual parts were confirmed in Freiburg format, we cloned them using the AgeI and SpeI site, creating a scar that also functions as a small linker.
Source
Phytochelatin and CBDcenA were synthesized from Geneart and cloned into the psB1C3 backbone.