Difference between revisions of "Part:BBa B0013:Design"

 
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===Design Notes===
 
===Design Notes===
  
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<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The      5' end cutoff was placed immediately after the TAA stop codon and the 3' end      cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Subsequently      mutations were introduced to make the terminator bi-directional (i.e., AAAAAA insertion on 5' side of the stem loop). Additional mutations were introduced on the 3' side of the stem      loop to increase the number of T's and eliminate any promoter -10 site that      might be present to avoid initiation of transcription of whatever is downstream.<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
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Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The      5' end cutoff was placed immediately after the TAA stop codon and the 3' end      cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Subsequently      mutations were introduced to make the terminator bi-directional (i.e., AAAAAA insertion on 5' side of the stem loop). Additional mutations were introduced on the 3' side of the stem      loop to increase the number of T's and eliminate any promoter -10 site that      might be present to avoid initiation of transcription of whatever is downstream.
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Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
  
  

Revision as of 01:07, 30 April 2006


TE from coliphage T7 (+/-)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Design Notes

Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Subsequently mutations were introduced to make the terminator bi-directional (i.e., AAAAAA insertion on 5' side of the stem loop). Additional mutations were introduced on the 3' side of the stem loop to increase the number of T's and eliminate any promoter -10 site that might be present to avoid initiation of transcription of whatever is downstream. 

Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.


Source

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References

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