Difference between revisions of "Part:BBa C0161:Experience"

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= Induction by the Supernatant of a LuxI Producing Culture =
 
= Induction by the Supernatant of a LuxI Producing Culture =
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The production of an inducer for the [AHL|3OC6-HSL] and [Part:BBa_C0062|LuxR] dependent promoter [Part:BBa_R0062|R0062] by LuxI could be shown using the supernatant of a culture containing ''E. coli'' cells constitutively expressing LuxI to induce the [AHL|3OC6-HSL] sensor construct in another culture.
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The production of an inducer for the [[AHL|3OC6-HSL]] and [[Part:BBa_C0062|LuxR]] dependent promoter [[Part:BBa_R0062|R0062]] by [[Part:BBa_C0161|LuxI]] could be shown using the supernatant of a culture containing ''E. coli'' cells constitutively expressing [[Part:BBa_C0161|LuxI]] to induce the [[AHL|3OC6-HSL]] sensor construct with the reporter sfGFP in another culture.
 
== Background information ==
 
== Background information ==
  

Revision as of 21:40, 20 October 2014

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Applications of BBa_C0161

User Reviews

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ETH Zurich 2014

Induction by the Supernatant of a LuxI Producing Culture

The production of an inducer for the 3OC6-HSL and LuxR dependent promoter R0062 by LuxI could be shown using the supernatant of a culture containing E. coli cells constitutively expressing LuxI to induce the 3OC6-HSL sensor construct with the reporter sfGFP in another culture.

Background information

Experimental Setup

Results

Figure 1: Dose-response curve LuxI producer supernatant The supernatant culture of E. coli cells in LB medium constitutively expressing LuxI was used in different dilutions with fresh LB medium as growth medium for E. coli cell containing a construct expressing sfGFP under a Plux promoter and constitutively expressing the regulator LuxR. Fluorescence/OD600 of sfGFP was measured 6 h after inoculation.



Antiquity

This review comes from the old result system and indicates that this part did not work in some test.

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