Difference between revisions of "Part:BBa C0161:Experience"
(→Results) |
(→Induction by the Supernatant of a LuxI Producing Culture) |
||
| Line 27: | Line 27: | ||
= Induction by the Supernatant of a LuxI Producing Culture = | = Induction by the Supernatant of a LuxI Producing Culture = | ||
| − | + | The production of an inducer for the [AHL|3OC6-HSL] and [Part:BBa_C0062|LuxR] dependent promoter [Part:BBa_R0062|R0062] by LuxI could be shown using the supernatant of a culture containing ''E. coli'' cells constitutively expressing LuxI to induce the [AHL|3OC6-HSL] sensor construct in another culture. | |
== Background information == | == Background information == | ||
Revision as of 21:38, 20 October 2014
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_C0161
User Reviews
UNIQ26a8f1c9d0c2aa83-partinfo-00000000-QINU
|
••••
ETH Zurich 2014 |
Induction by the Supernatant of a LuxI Producing CultureThe production of an inducer for the [AHL|3OC6-HSL] and [Part:BBa_C0062|LuxR] dependent promoter [Part:BBa_R0062|R0062] by LuxI could be shown using the supernatant of a culture containing E. coli cells constitutively expressing LuxI to induce the [AHL|3OC6-HSL] sensor construct in another culture. Background informationExperimental SetupResults Figure 1: Dose-response curve LuxI producer supernatant The supernatant culture of E. coli cells in LB medium constitutively expressing LuxI was used in different dilutions with fresh LB medium as growth medium for E. coli cell containing a construct expressing sfGFP under a Plux promoter and constitutively expressing the regulator LuxR. Fluorescence/OD600 of sfGFP was measured 6 h after inoculation.
|
|
Antiquity |
This review comes from the old result system and indicates that this part did not work in some test. |
UNIQ26a8f1c9d0c2aa83-partinfo-00000003-QINU