Difference between revisions of "Part:BBa K1321366:Design"

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===Source===
 
===Source===
  
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CBDcex comes from ''Cellulomonas fimi'', and sfGFP is a modified super-folding mutant of GFP.  
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CBDcex comes from ''Cellulomonas fimi'', and sfGFP is a modified super-folding mutant of GFP. Our sfGFP part was an improvement of BBa_I746909, wherein Freiburg sites were added. Please see BBa_K1321337 for more info on this part. CBDcex was requested from the registry, as it was submitted by the Freiburg team as BBa_K863101.
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The T7 vector was made by inverse PCR of BBa_I746909, and ligated. Please see our BBa_ K1321338 for more information on this part.
  
 
===References===
 
===References===

Revision as of 23:41, 21 October 2014

CBDcex fused to sfGFP with T7 promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 53
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 401


Design Notes

This part was made from the composite parts that we submitted, and fused by Biobrick and Freiburg cloning. SfGFP was fused as a reporter for our CBD binding assay, and may have uses for future teams as an easily quantifiable reporter.

Source

CBDcex comes from Cellulomonas fimi, and sfGFP is a modified super-folding mutant of GFP. Our sfGFP part was an improvement of BBa_I746909, wherein Freiburg sites were added. Please see BBa_K1321337 for more info on this part. CBDcex was requested from the registry, as it was submitted by the Freiburg team as BBa_K863101.

The T7 vector was made by inverse PCR of BBa_I746909, and ligated. Please see our BBa_ K1321338 for more information on this part.

References