Difference between revisions of "Part:BBa J37015"

Revision as of 14:23, 30 October 2006

Prey Molecule Generator (AHL) + GFP Reporter

Prey Genotype + Reporter

Usage:

This part was created by iGEM@Imperial 2006 to be used as the prey part in their 2006 project [http://openwetware.org/wiki/IGEM:IMPERIAL/2006 'Engineering a Molecular Predation Oscillator'] to generate a the production of Acyl-Homoserine Lactone (a quorum sensing molecule) using a positive feedback loop.

Biology:

The positive feedback loop relies on leaky expression of LuxR by the tetR promoter which causes production of luxR. AHL already present in the cell forms a complex with luxR and lux pR promoter, expressing LuxI and GFP. LuxI enzymatically produces AHL. GFP can be used to monitor transcription of LuxI as a check for the functionality of the part.
The behaviour of this part was successfully modelled, generating a [http://openwetware.org/wiki/IGEM:IMPERIAL/2006/project/Oscillator/project_browser/Prey_Construct/Modelling transfer curve of AHL vs. Time].

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1724
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1004
Illegal BsaI.rc site found at 2400

@@ Line 10: / Line 10: @@
 '''Biology:'''
-*The positive feedback loop relies on leaky expression of the tetR promoter which causes production of luxR. AHL already present in the cell forms a complex with luxR and lux pR promoter, expressing luxI and GFP. LuxI enzymatically produces AHL.
+*The positive feedback loop relies on leaky expression of LuxR by the tetR promoter which causes production of luxR. AHL already present in the cell forms a complex with luxR and lux pR promoter, expressing LuxI and GFP. LuxI enzymatically produces AHL. GFP can be used to monitor transcription of LuxI as a check for the functionality of the part.
 *The behaviour of this part was successfully modelled, generating a [http://openwetware.org/wiki/IGEM:IMPERIAL/2006/project/Oscillator/project_browser/Prey_Construct/Modelling transfer curve of AHL vs. Time].