Difference between revisions of "Part:BBa K1331003"

Revision as of 04:42, 19 October 2014

RhlI Acyl-homoserine-lactone synthase from P.aeruginosa SQ6

This part encodes rhlI, an acyl-homoserine-lactone synthase in Pseudomonas aeruginosa. The EcoRI(at 250) and PstI(at 692) restriction sites have been mutated to meet BioBrick RFC[10] requirements without changing the amino acid sequence.

Usage and Biology

The sequence we provide here includes the whole coding sequence and a part of upstream noncoding sequence of rhlI.

RhlI is an acyl-homoserine-lactone synthase required for the synthesis of autoinducer molecules C4-HSL, which together with rhlR positively regulate the promoter of rhlAB.

The noncoding sequence included in this part is for composition with our part BBa_K1331002 . See our composite part rhlRI ([http://https://parts.igem.org/Part:BBa_K1331007 BBa_K1331007]).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 89
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 221

@@ Line 9: / Line 9: @@
 The sequence we provide here includes the whole coding sequence and a part of upstream noncoding sequence of rhlI.
-RhlI is an acyl-homoserine-lactone synthase required for the synthesis of BHL (N-butanoyl-L-homoserine lactone), and HHL (N-hexanoyl-L-homoserine lactone) autoinducer molecules which bind to RhlR. RhlI is required for the synthesis of autoinducer molecules C4-HSL.Together with rhlR positively regulates the promoter of rhlAB.
+RhlI is an acyl-homoserine-lactone synthase required for the synthesis of autoinducer molecules C4-HSL, which together with rhlR positively regulate the promoter of rhlAB.
 The noncoding sequence included in this part is for composition with our part [https://parts.igem.org/Part:BBa_K1331002 BBa_K1331002]