Difference between revisions of "Part:BBa K1331006"

Revision as of 04:12, 19 October 2014

Modified rhlAB coding sequence from Pseudomonas aeruginosa SQ6

This part encodes both the subunit A and B of rhamnosyltransferase I. It is composited with BBa_K1331001 and BBa_K1331004.

It includes both the coding sequence of RhlA, RhlB and the whole noncoding sequence between RhlA and RhlB.

The purpose of remaining this noncoding sequence is to ensure the normal expression of rhamnosyltransferase I.

This part encodes both the subunit A and B of rhamnosyltransferase I, which is the essential protein for rhamnolipid synthesis.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 69
Illegal BamHI site found at 629
Illegal XhoI site found at 805
Illegal XhoI site found at 2099
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 992
Illegal NgoMIV site found at 1713
Illegal NgoMIV site found at 1826
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 294
Illegal BsaI site found at 1342
Illegal BsaI.rc site found at 478

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1331006 short</partinfo>
 This part encodes both the subunit A and B of rhamnosyltransferase I. It is composited with [https://parts.igem.org/Part:BBa_K1331001 BBa_K1331001] and [https://parts.igem.org/Part:BBa_K1331004 BBa_K1331004].
-The part includes both the coding sequence of RhlA, RhlB and the whole noncoding sequence between RhlA and RhlB.
+It includes both the coding sequence of RhlA, RhlB and the whole noncoding sequence between RhlA and RhlB.
 The purpose of remaining this noncoding sequence is to ensure the normal expression of rhamnosyltransferase I.